Original Research

Comparison of progesterone assay by chemiluminescence or radioimmunoassay for clinical decision-making in canine reproduction

Johan O. Nöthling, Kurt G.M. De Cramer
Journal of the South African Veterinary Association | Vol 90 | a1730 | DOI: https://doi.org/10.4102/jsava.v90i0.1730 | © 2019 Johan O. Nöthling, Kurt G.M. De Cramer | This work is licensed under CC Attribution 4.0
Submitted: 31 August 2018 | Published: 22 October 2019

About the author(s)

Johan O. Nöthling, Department of Production Animal Studies, Section of Reproduction, Faculty of Veterinary Science, Onderstepoort, South Africa
Kurt G.M. De Cramer, Department of Production Animal Studies, Section of Reproduction, Faculty of Veterinary Science, Onderstepoort, South Africa; and, Rant en Dal Animal Hospital, Mogale City, South Africa


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Abstract

The Coat-A-Count® radioimmunoassay has been long and widely used to determine the concentration of progesterone in serum or plasma of bitches (progRIA), but was discontinued in 2014. The Immulite® 1000 LKPG1 chemiluminescence immunoassay has gained prominence since 2003 to determine the concentration of progesterone in serum of bitches, but the assay changed in 2012 (Immulite® 1000 LKPW1). This study assessed the feasibility of using Immulite® 1000 LKPW1 (progImm) to estimate the time of clinically relevant events during oestrus and compared progRIA and progImm 2 and 3 days after the first or only day of the luteinizing hormone surge (LH1). ProgImm first exceeded 5.1 nmol/L on the same day that progRIA first exceeded 6 nmol/L, a proxy for the occurrence of the LH surge, or the day before in 28 of 31 (90%) of oestrous periods. ProgImm first exceeded 13.6 nmol/L on the same day that progRIA first exceeded 16 nmol/L (a proxy for the day of ovulation) or the day before in 34 of 35 (97%) oestrous periods. ProgImm first exceeded 5.4 nmol/L on LH1 or the day before in 24 of 25 (95%) of oestrous periods. The median of progImm 2 days after LH1 was 1.2 nmol/L lower than the 10.7 nmol/L of progRIA (p = 0.001). The mean of progImm 3 days after LH1 was 2.2 nmol/L lower than the 19.0 nmol/L of progRIA (p < 0.001). In conclusion, the days on which progImm first exceeded 5.1 nmol/L, 13.6 nmol/L and 5.4 nmol/L effectively estimate the days on which progRIA reached 6 nmol/L or 16 nmol/L or LH1.

Keywords

progesterone; bitch; radioimmunoassay; Coat-A-Count; Immulite; luteinizing hormone; LH

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