Original Research

Isolation of Bartonella henselae from a serologically negative cat in Bloemfontein, South Africa

A-M Pretorius, P.J. Kelly, R.J. Birtles, D. Raoult
Journal of the South African Veterinary Association | Vol 70, No 4 | a785 | DOI: https://doi.org/10.4102/jsava.v70i4.785 | © 1999 A-M Pretorius, P.J. Kelly, R.J. Birtles, D. Raoult | This work is licensed under CC Attribution 4.0
Submitted: 12 July 1999 | Published: 12 July 1999

About the author(s)

A-M Pretorius,
P.J. Kelly,
R.J. Birtles,
D. Raoult,

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Abstract

Sera collected from apparently healthy 6-12-month-old cats (n = 31) presented to the Society for the Prevention of Cruelty to Animals Veterinary Clinic in Bloemfontein for neutering were tested for antibodies reactive to Bartonella henselae (Houston-1 strain) by indirect fluorescent antibody testing. Whole blood collected from the cats was used in isolation experiments and subsequent identification of Bartonella species was based on comparison of the nucleotide base sequence of polymerase chain reaction-amplified citrate synthase gene fragments. While none of the cats had antibodies reactive with B. henselae at titres > 1/64, an organism with a partial citrate synthase gene sequence identical to that of B. henselae (Houston-1) was isolated from 1 cat.

Keywords

Bartonella Henselae; Cats; Isolation; Serological Survey; South Africa

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